Table 1. Ste2 variants and their dose-response sensitivity to either pheromone.

Name	Protein mutations	Scer EC50 (nM)	Klac EC50 (nM)
WT	NA	8.74±2.2	726±240
S1	I82N, N216D, Y266F	4.36±0.42	19.1±6.3
S2	V280I	5.90±1.2	107±2.5
S3	S267C,V280D, K358R,T414M	1.73±0.096	6.24±1.4
S4	N216S	8.45±0.11	24.1±7.6
S5	S267R, T282S	3.30±0.19	86.6±28
S6	M54V, A62T, M69L, G115R	7.81±0.11	1500±620
T1	Y30H, K358*	0.375±0.14	80.4±12
T2	T78M, A336D, K337P, S338E, S339*	0.909±0.20	100±8.9
T3	G237D, F312L, R350*	0.758±0.0049	84.7±20
F1	N25D, K202T, T309N, A397E, Fs401	0.649±0.27	224±1.8

NA, not applicable; WT, wild type.

Asterisks designate a premature stop codon while ‘Fs' designates a frameshift mutation.

Values represent the mean±s.e.m.