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. 2016 Aug 3;7:12426. doi: 10.1038/ncomms12426

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Copyright © 2016, The Author(s)

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(a) List of proteins identified in CGN immunoprecipitate by mass spectroscopy. Proteins known to interact as a complex are highlighted together. (b) Schematic representation of the WAVE/Scar complex. (c–i) MDCK cells were embedded in 3D Matrigel and allowed to grow for 24 h. Cells were then fixed and stained with phalloidin-Alexa594 (g–i; red), anti-CGN (f,g,i), anti-acetylated tubulin (c; red and h; green) or anti-Nap1 (c–e; green) antibodies. In (f), cell is expressing Arp3-GFP. White arrows identify structures as labelled. Consecutive images without an individual letter label show the same cell.