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. 2016 Aug 3;7:12426. doi: 10.1038/ncomms12426

Figure 6. Rac1 is required for the gp135 targeting during apical lumen formation.

Figure 6

(a–d) MDCK cells transiently expressing GFP-Abi2 were embedded in 3D Matrigel and allowed to grow for 24 h. Where indicated, cells were treated with 200 μM Rac1 inhibitor (b,d). Cells (a,b) were then fixed and stained with anti-acetylated tubulin (red) antibodies. White arrows (a,b) point to the midbody. (e–h) MDCK cells were embedded in 3D Matrigel and allowed to grow for 24 h. Where indicated, cells were treated with 200 μM Rac1 inhibitor (g,h). Cells were then fixed and stained with anti-gp135 (green) and anti-CGN (red) antibodies. White arrow in (e) points to the midbody. White arrow in (g) points to misplaced CGN. (i,j) MDCK cells were embedded in 3D Matrigel and allowed to grow for 2 days. Cells were treated with 200 μM Rac1 inhibitor for the first 12 h of the incubation. Cells were then fixed and stained with anti-gp135 (green) and anti-CGN (red) antibodies. White arrow in (j) points to misplaced CGN. Consecutive images without an individual letter label show the same cell.