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. 2016 Aug 8;7:245. doi: 10.3389/fphar.2016.00245

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Copyright © 2016 Banerjee, Chakraborty, Ghosh, Raha, Sen and Jana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Curcumin and resveratrol prevent B(a)P induced caspase dependent testicular germ cell apoptosis. (A) Light microscopic image of isolated testicular germ cells at ×400 magnification. (B) DNA ladder assay of isolated germ cells. (C) Western blot analysis for cleaved caspase 3, cleaved PARP, cleaved Caspase 8 and cleaved Caspase 9. β Actin was used as the internal loading control. The numerical values indicated below the immunoblots are the densitometric analysis values expressing mean ± SEM of relative arbitrary units of the bands for three immunoblots were conducted with separate experiments in each group. One-way ANOVA was followed by Dunnett multiple comparison test. The level of significance was set at (^∗∗∗P < 0.001); (^∗∗P ≤ 0.01–0.001); (^∗P ≤ 0.01–0.05) in respect with the control.