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Western blot analysis of pCABS1 from testis and epididymis. 20 µg proteins extracted from testis, noncapacitated sperm, or capacitated sperm were separated on 10% SDS-PAGE. (A) Coomassie Brilliant Blue-staining. (B) Western blot analysis with anti-pCABS1 antiserum. Lane 1: Testis; lane 2: Noncapacitated sperm; lane 3: Capacitated sperm; and lane 4: bovine serum albumin (BSA) as a negative control. M: Molecular marker (kilodalton; kDa).
