Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 8;65(3):253–265. doi: 10.1538/expanim.15-0104

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2016 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

PMC Copyright notice

Fig. 8. — Effect of anti-pCABS1 antiserum on the acrosome reaction of porcine sperm. (A–D) Representative photos of four fluorescence patterns stained with FITC-conjugated peanut agglutinin (PNA)-lectin and propidium iodide (PI) for the assessment of acrosome status and sperm viability. (A, B) Live sperm without PI staining. (C, D) Dead sperm showing nuclear red PI fluorescence. (A) Acrosome-reacted sperm with acrosomal cap and uniform green fluorescence FITC-PNA. (B) Acrosome-unreacted sperm with no staining of the acrosomal cap. Scale bars: 5 µm. (E) Percentage of acrosome-reacted live sperm after incubation with anti-pCABS1 diluted 1:200 and 1:50 (treated) vs. incubation with the neutralized anti-pCABS1 antiserum diluted 1:50 (control). The bar chart demonstrates mean percentage ± SEM (n=200). *Significantly different from control sperm (P<0.05).