FIG. 1.

DGGE analysis showing profiles of healing (n = 8) and nonhealing wounds (n = 10) of 16S rRNA amplified directly from chronic wound tissue. Lanes are identified by patient number. “S” is an in-house four-strain standard comprising Peptostreptococcus sp. (A), Pseudomonas sp. (B), Proteus mirabilis (C), and Micrococcus sp. (D). Bands amplified from the tissues are labeled a to d and correlate to bands that were excised and sequenced. The arrow indicates an apparently identical band found in all tissue samples and identified from sequence analysis to be Pseudomonas sp.