TABLE 1.

Cultured bacterial isolates from swabs and biopsies from individual patients with healing chronic venous leg ulcers and DGGE analysis of the so-called unculturable population from the same wounds

Patient	Wound surface (swab)a	Wound bed (biopsy)b	DGGE (PCR-cloning/sequencing)c
12	Coagulase-negative Staphylococcus sp. and Staphylococcus aureus	Coagulase-negative Staphylococcus sp.	Pseudomonassp. (a) (Pseudomonas putida subgroup) and Salmonellasp. (b)
19	Coagulase-negative Staphylococcus sp., Micrococcus sp. and Streptococcus sp.	Coagulase-negative Staphylococcus sp. and Micrococcus sp.	Stenotrophomonas maltophilia, (a) Paenibacillus sp., and Pseudomonassp. (b) (Pseudomonas putida subgroup)
24	Eubacteriumsp.	Staphylococcus aureus, coagulase-negativeStaphylococcus sp., and Peptostreptococcussp.	Staphylococcus sp. (a) (Staphylococcus aureus subgroup) and Pseudomonassp. (b) (Pseudomonas putida subgroup)
26	Pseudomonassp. and gram-negative nonfermenter	Staphylococcus aureus, coagulase-negativeStaphylococcus sp., and Micrococcussp.	Sphinogomonassp. (a) and Staphylococcus sp. (b) (Staphylococcus aureus subgroup)
28	Proteus mirabilis, Staphylococcus aureus, coagulase-negativeStaphylococcus sp., Peptostreptococcussp., Bordetellasp., and Stenotrophomonas maltophilia	Proteus mirabilis and Bacteroidessp.	Sphinogomonassp. (a) and Peptoniphilus sp. (b)
48	Coagulase-negative Staphylococcus sp., Pseudomonas sp., Peptostreptococcussp., Eubacterium sp., and Aerococcussp.	Coagulase-negative Staphylococcus sp., Pseudomonas sp., Streptococcussp., and Proteus mirabilis	Pseudomonas sp. (a) (Pseudomonas aeruginosa subgroup) and Streptococcus dysgalactiae (b)
49	Coagulase-negative Staphylococcussp., gram-negative nonfermenter, and Aerococcussp.	Pseudomonassp. and Salmonellasp.	Corynebacterium striatum, unidentified β-proteobacteriumd, and-e (c)
53	Coagulase-negative Staphylococcus sp., Pseudomonas sp., Peptostreptococcus magnus, and Streptococcussp.	Coagulase-negative Staphylococcus sp., Pseudomonas sp., Peptostreptococcus magnus, and Staphylococcus aureus	-

^aUnderlined species in this column were isolated only from the swab and not from the biopsy.

^bUnderlined species in this column were isolated only from the biopsy and not from the swab.

^cUnderlined species in this column were identified only from PCR cloning-sequencing and not cultured from the wound, i.e., uncultured or unculturable. Where clones could not be identified to a single specific species, the closest related subgroup is given in parentheses. Designations (a), (b), (c), and (d) refer to the bands excised from the DGGE gel in Fig. 1.

^dThe accession no. of the sequence showing closest identity is AF467355.

^eIdentified as the Taq polymerase contaminant, Thermus sp.