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. 2016 Mar 14;65(3):275–283. doi: 10.1538/expanim.15-0116

Fig. 1.

Fig. 1.

Generation of mutant mice using crRNA/tracrRNA in the Bcr locus. (A) The design of the crRNA in the mouse Bcr locus. The genomic structure is indicated at the top. The black bars and boxes show introns and exons, respectively. Blue letters and red lines show specific target sequences and common sequences of crRNA, respectively. The letters with yellow boxes designate PAM sequences. The blue curved lines indicate tracrRNA. Longitudinal blue bars show hydrogen bonds. (B) Representative electropherograms obtained from the sequencing analysis of wild- type, monoallelic, biallelic, and mosaic mutant embryos injected with crRNA1/tracrRNA/Cas9WT. The target sequence of crRNA1 is indicated with a black line. The PAM sequence is indicated with a yellow box. (C) The proportions of the genotypes of embryos obtained by crRNA1/tracrRNA/Cas9WT injection.