Table 1. A summary of the mutant mice produced using crRNA/tracrRNA.

Denatured	Trial	Injected/2-cell	Transferred	Genotyped	Mutated	Monoallelic	Biallelic	Mosaic
untreated	1	58/38	36	16	11	2	3	6
	2	63/26	26	20	9	3	1	5
	total	121/64	62	36	20 (55.6)	5 (25.0)	4 (20.0)	11 (55.0)
denatured	1	51/48	36	22	9	3	4	2
	2	71/33	32	18	9	2	6	1
	total	122/81	68	40	18 (45.0)	5 (27.8)	10 (55.6)	3 (16.7)

The numbers in parentheses in the “Mutated” columns represent the percentages calculated from the number of mutants relative to the number of genotyped embryos. The numbers in parentheses in the “Monoallelic”, “Biallelic” and “Mosaic” columns represent the percentages calculated from the number of each type relative to the number of “Mutated”.