Table 2. A summary of the mutant mice produced using crRNA/tracrRNA with Cas9 mutants.
| Locus | Cas9 | Trial | Injected/2-cell | Transferred | Genotyped | Mutated | Monoallelic | Biallelic | Mosaic |
|---|---|---|---|---|---|---|---|---|---|
| Bcr | nCas9 | 1 | 56/44 | 30 | 24 | 1 | 0 | 0 | 1 |
| 2 | 38/23 | 23 | 8 | 1 | 1 | 0 | 0 | ||
| 3 | 42/33 | 33 | 15 | 10 | 5 | 2 | 3 | ||
| total | 136/100 | 86 | 47 | 12 (25.5) | 6 (50.0) | 2 (16.7) | 4 (33.3) | ||
| fCas9 | 1 | 36/26 | 26 | 21 | 0 | 0 | 0 | 0 | |
| 2 | 43/24 | 24 | 13 | 7 | 1 | 3 | 3 | ||
| 3 | 39/26 | 26 | 11 | 1 | 0 | 0 | 1 | ||
| 4 | 39/30 | 30 | 14 | 9 | 5 | 1 | 3 | ||
| total | 157/106 | 106 | 59 | 17 (28.8) | 6 (35.3) | 4 (23.5) | 7 (41.2) | ||
| Abl1 | nCas9 | 1 | 134/118 | 60 | 39 | 8 (20.5) | 2 (25.0) | 1 (12.5) | 5 (62.5) |
| fCas9 | 1 | 145/115 | 60 | 26 | 10 (38.5) | 2 (20.0) | 6 (60.0) | 2 (20.0) | |
The numbers in parentheses in the “Mutated” columns represent the percentages calculated from the number of mutants relative to the number of genotyped embryos. The numbers in parentheses in the “Monoallelic”, “Biallelic” and “Mosaic” columns represent the percentages calculated from the number of each type relative to the number of “Mutated”.