. 2016 Mar 14;65(3):275–283. doi: 10.1538/expanim.15-0116

Table 3. A summary of mutation efficiencies by microinjection of sgRNA and crRNA/tracrRNA with various Cas9s.

Locus	Guide RNA	Cas9 variants	Mutation rate	Reference
Bcr	sgRNA	WT	66.7%	[7]
		nCas9	33.3%
		fCas9	61.7%

	crRNA/tracrRNA	WT	55.6%	the present study
		nCas9	27.7%
		fCas9	28.8%

Abl1	sgRNA	nCas9	N/A	[7]
	sgRNA	fCas9	36.0%	[7]

	crRNA/tracrRNA	nCas9	23.1%	the present study
	crRNA/tracrRNA	fCas9	38.5%	the present study