Table 1. P. larvae isolates analyzed in this study.
| Isolate | Source | Site of occurrence | Year of the AFB outbreak | Spores in a diseased brood | Pigmented colonies | Hemolysis | Spore formation in vitroa) | Average days needed to form spores in vitroa) | Genotyping results | |
|---|---|---|---|---|---|---|---|---|---|---|
| ERIC | MLST | |||||||||
| N | Diseased brood | Seto city | 2012 | + | + | − | + | 10 days | II | ST10 |
| O | Diseased brood | Ichinomiya city | 2013 | + | + | − | − | II | ST10 | |
| KH | Diseased brood | Inuyama city | 2014 | + | + | − | − | II | ST10 | |
| IK | Diseased brood | Kasugai city | 2014 | + | + | − | − | II | ST10 | |
| I | Diseased brood | Inazawa city | 2013 | + | − | − | + | 3.6 days | I | ST15 |
| SK | Diseased brood | Seto city | 2014 | + | − | − | + | 4 days | I | ST2 |
| KM | Diseased brood | Aisai city | 2014 | + | − | − | − | I | ST15 | |
a) The formation of spores was examined on J´-agar at 37°C in air plus 5% CO2 for 14 days.