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. 2016 Aug 4;7:12381. doi: 10.1038/ncomms12381

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Average rate of phosphorylation of RsbR (a) RsbS (b) and RsbT (c) in a RsbR/S/T mixture (ligation/oxidation states of RsbR: Fe^II-O₂, red; Fe^II unligated, black). All experiments were performed at least in triplicate, and mean and s.d. values are plotted for each time point. Phosphorylation of RsbS was not detected above background levels when Fe^II-O₂ RsbR was included in the reaction mixture and therefore did not show any time-dependent kinetics (variability in the RsbS Fe^II-O₂ plots is due to normalization within the background signals).