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. 2016 Aug 8;6:31086. doi: 10.1038/srep31086

Figure 3. Genetic knockdown of HDAC6 reduces TGF-β1-activation of Notch Signaling in A549 cells.

Figure 3

(A) A549 cells transiently transfected with siRNA targeting HDAC6, Notch1, or non-specific control were serum starved and treated with TGF-β1 for 24 hours. Whole cell lysate protein levels of HDAC6, Notch1, HEY-1, and HES-1 were examined by western analysis (separate blots provided of the same experiment). (B,C) A549 cells treated as in A, except RNA was isolated and quantitative RT-PCR was carried out for HEY-1 (B) and HES-1 (C). (D,E) Densitometry analysis of protein levels of HEY-1 (D) and HES-1 (E) from three independent experiments. For qPCR analysis data presented as mean +/− SEM of triplicate wells and are representative of three independent experiments statistically analyzed using one-way ANOVA. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the relative control.