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. 2016 Aug 8;6:31086. doi: 10.1038/srep31086

Figure 5. HSP90 inhibition effect on TGF-β1-induced Notch signaling in A549 cells.

Figure 5

(A–C) Serum-starved A549 cells were pre-treated for six hours with either 1 μM or 10 μM 17-AAG or an equivalent volume of DMSO for six hours before being exposed to TGF-β1 (2.5 ng/ml) for 24 hours. RNA was isolated and quantitative RT-PCR was carried out for HEY-1, HES-1, and PAI1 transcripts. (D) A549 cells transiently transfected with siRNA targeting HDAC6, Notch1, or non-specific control were serum starved and treated with TGF-β1 for 24 hours. RNA was isolated and Quantitative RT-PCR was carried out for HMOX1 transcripts. The –fold change of each transcript was obtained by setting the value of the untreated cells to 1. Data presented as mean +/− SEM of triplicate wells and are representative of three independent experiments (A–C) and D as mean +/− STD of triplicate wells from a single independent experiment statistically analyzed using one-way ANOVA. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the relative control.