TABLE 1.
Primers used in this study
| Primer type and name | Sequence (5′-3′) |
|---|---|
| Adapters | |
| PstI | GACTGCGTAGGTGCA |
| PstI | CCTACGCAGTCTACGAG |
| MseI | GACGATGAGTCCTGAG |
| MseI | TACTCAGGACTCAT |
| Internal | |
| region1F | CGCACCAAAAGGCACACTAATC |
| region1R | CGTCGTCACCATTCAGGAACTC |
| region2F | GGCTCGCCGAACTACTTGT |
| region2R | CTCGAAACAACGGTGACAGA |
| region3F | GCGGAAAGTCACACTGCTGATGC |
| region3R | CGGTCTATTCGATCCCCACCTTG |
| region4F | CGAGTTGTCCTGGGGTTTTGG |
| region4R | CGAAAGTCACCGCATCCACG |
| Primer walking | |
| PW6 | CGATCGGCTTTACACCAC |
| PW1106 | AGAAGCCGGCAACACTCGCC |
| PW952 | GCTTAGGAGGAGGTGGCTGATAATC |
| PW2485 | CCCCAACCCTGTTCGGTATG |
| PW2465 | GCATACCGAACAGGGTTGGG |
| PW3741 | GTGGCACTGGTGGACATTTTG |
| PW3706 | GTCCATCGCACACCTCAAAATG |
| PW5360 | CGCTCAGCATCATCGTGAAGTAG |
| Positive control | |
| F1-PC-190 | CAACCTCAAACCCGAATAC |
| B1-PC-190 | CTTGCTGATGGTGGTCTG |
| IS900 PCR | |
| P90 | GAAGGGTGTTCGGGGCCGTC |
| P91 | GAGGTCGATCGCCCACGTGAC |