TABLE 2.
Results of PCR assays of all isolates with internal primer sets
Isolate | Sourcea | Result obtained with diagnostic primer setb based on:
|
|||
---|---|---|---|---|---|
Region 1 | Region 2 | Region 3 | Region 4 | ||
109 | W | + | + | + | + |
126 | W | + | + | + | + |
129 | W | + | + | + | + |
130 | W | + | + | + | + |
132 | W | + | + | + | − |
135 | W | + | + | + | + |
136 | W | + | + | + | + |
139 | W | + | + | + | + |
140 | W | + | + | + | + |
141 | W | + | + | + | + |
142 | W | + | + | + | + |
144 | W | + | + | + | + |
145 | W | + | + | + | + |
148 | W | + | + | + | + |
150 | W | + | + | + | + |
151 | W | + | + | + | + |
155 | W | + | + | + | + |
156 | W | + | + | + | + |
160 | W | + | + | + | + |
ATCC 35716 | A | − | − | − | + |
Mac 104 | N | − | − | − | − |
ATCC 19698 | A | + | + | + | + |
Isolate source: W, UWDL; N: University of Nebraska Barletta Laboratory; A, ATCC.
Outcome of PCR with primers derived from sequences of unique bands present on AFLP gels: +, presence of a PCR product; −, absence of a PCR product.