TABLE 3.
Results of PCR assays of NVSL isolates obtained with diagnostic primer sets
| Isolate | Type of isolatea | Sheddingb | Result obtained with internal primer setc based on:
|
|||
|---|---|---|---|---|---|---|
| Region 1 | Region 2 | Region 3 | Region 4 | |||
| 1 | MparaTb | H | + | + | + | + |
| 2 | N | N | − | − | − | − |
| 3 | MparaTb | L | + | + | − | + |
| 4 | MparaTb | M | + | + | + | − |
| 5 | MparaTb | H | + | + | + | + |
| 6 | N | N | − | − | − | − |
| 7 | MparaTb | M | + | − | − | − |
| 8 | MparaTb | L | + | + | + | + |
| 9 | MparaTb | L | + | − | − | − |
| 10 | MparaTb | H | + | + | + | + |
| 11 | N | N | − | − | − | − |
| 12 | Invalid | Invalid | + | − | − | − |
| 13 | Invalid | Invalid | − | − | − | − |
| 14 | MparaTb | L | + | + | + | − |
| 15 | MparaTb | H | − | − | − | − |
| 16 | N | N | − | − | − | − |
| 17 | MparaTb | L | + | + | + | − |
| 18 | MparaTb | L | + | + | + | + |
| 19 | MparaTb | H | + | + | + | − |
| 20 | MparaTb | H | + | − | − | − |
| 21 | N | N | − | − | − | − |
| 22 | MparaTb | M | + | − | + | − |
| 23 | N | N | − | − | − | − |
| 24 | MparaTb | H | − | − | + | − |
| 25 | MparaTb | H | + | + | + | + |
a
Results of the double-blind study as provided by NVSL.
b
NVSL isolates were either high (H), moderate (M), or low (L) shedders. Negative (N) samples and invalid samples are also shown.
c
A plus sign denotes the presence of a PCR product; a minus sign denotes the absence of a PCR product.