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. 2016 Aug 8;6:31217. doi: 10.1038/srep31217

Figure 2. Fluorescent properties of MAO-Red-1 and MAO-Red-2.

Figure 2

(a) Fluorescence intensity of MAO-Red-1 before and after reaction with MAO-A and MAO-B. Inserted plot: fluorescence kinetic assay of MAO-Red-1 (10 μM) after the addition of MAO (10 μg mL−1); (b) The signal-to-background ratio of MAO-Red-1 and MAO-Red-2 after reactions with MAO-A, MAO-B, subtilisin, BSA and inhibited MAO; [protein] = 0.01–0.15 mg mL−1. The data were recorded in enzyme assay buffer (100 mM HEPES, pH = 7.4 with 5% glycerol and 1% DMSO) at 37 °C (λex/λem = 420/664 nm); (c) Normalized fluorescent spectra of MAO-Red-1, MAO-Red-2 and DCPO in the solid state; (d) Paper imaging of MAO-Red-1, MAO-Red-2 and DCPO under 365 nm UV light.