Figure 4. The Confocal fluorescence images of MAO-Red-1 in HeLa and HepG2 cells with or without the presence of MAO-B inhibitor Pargyline.
(a,e,i,m) Bright field images of HeLa and HepG2 cells incubated with MAO-Red-1 (5 μM) for 1 h; (b,f,j,n) fluorescence images from Green channel (λex = 405 nm); (c,g,k,o) fluorescence images from red channel (λex = 405 nm); (e–h,m–p) fluorescence images of cells that was incubated with pargyline for 2 h followed by 1 h incubation of MAO-Red-1. The fluorescence images were collected at 420–520 nm (green channel) and 630–700 nm (red channel), respectively.
