TABLE 3.
DNA sequence identities between the streptococcal tuf sequencesa
| Species no.b | Species name | % Identity with species no.b:
|
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | ||
| 1 | S. acidominimus ATCC 51726 | 91.6 | 90.0 | 90.3 | 90.0 | 90.1 | 89.5 | 89.5 | 89.6 | 89.1 | 92.2 | 90.1 | 89.9 | 87.9 | 89.8 | 88.6 | 89.1 | 90.4 | 88.3 | 88.9 | 89.2 | 90.5 | 90.5 | 89.2 | 89.4 | 91.2 | 90.1 | 90.7 | 82.4 | 76.3 | 77.5 | 78.6 | |
| 2 | S. agalactiae ATCC 13813 | 90.2 | 91.6 | 92.4 | 91.3 | 89.4 | 93.4 | 89.8 | 93.0 | 92.1 | 91.3 | 92.1 | 91.2 | 88.2 | 92.2 | 89.0 | 92.6 | 93.0 | 93.2 | 91.9 | 92.8 | 90.1 | 93.0 | 91.6 | 88.6 | 91.6 | 93.0 | 92.5 | 81.7 | 76.9 | 77.9 | 77.9 | |
| 3 | S. anginosus ATCC 33397 | 90.2 | 88.2 | 92.8 | 98.0 | 90.0 | 90.5 | 89.8 | 90.4 | 88.8 | 90.1 | 91.3 | 97.2 | 90.0 | 91.7 | 90.4 | 91.5 | 91.3 | 89.0 | 91.6 | 90.5 | 91.2 | 91.5 | 91.2 | 89.4 | 89.5 | 91.2 | 91.2 | 81.7 | 76.1 | 78.8 | 77.4 | |
| 4 | S. bovis ATCC 33317 | 88.9 | 89.5 | 92.2 | 92.1 | 90.9 | 92.0 | 90.0 | 90.5 | 89.8 | 90.4 | 91.5 | 92.0 | 89.2 | 92.0 | 90.8 | 90.8 | 91.6 | 88.6 | 91.1 | 90.1 | 91.9 | 92.0 | 91.1 | 90.0 | 91.1 | 91.1 | 91.5 | 82.8 | 75.0 | 77.8 | 77.0 | |
| 5 | S. constellatus ATCC 27823 | 89.5 | 86.9 | 96.7 | 90.2 | 89.4 | 90.8 | 89.1 | 89.9 | 88.7 | 89.8 | 90.8 | 97.8 | 90.1 | 91.2 | 90.1 | 90.9 | 91.3 | 89.2 | 91.1 | 89.5 | 90.5 | 91.5 | 90.7 | 88.4 | 89.0 | 90.5 | 91.2 | 81.6 | 76.0 | 78.7 | 77.8 | |
| 6 | S. criceti ATCC 19642 | 88.9 | 88.2 | 90.2 | 90.9 | 89.5 | 89.4 | 96.7 | 87.8 | 86.9 | 90.8 | 89.0 | 89.1 | 87.6 | 88.4 | 89.6 | 87.8 | 89.5 | 86.2 | 87.9 | 87.1 | 91.2 | 90.8 | 89.5 | 96.2 | 88.6 | 87.9 | 90.8 | 81.1 | 73.5 | 77.7 | 76.5 | |
| 7 | S. cristatus ATCC 51100 | 86.9 | 93.5 | 88.2 | 87.6 | 86.9 | 87.6 | 89.2 | 92.5 | 90.7 | 91.2 | 95.0 | 90.7 | 87.5 | 95.0 | 88.3 | 95.5 | 94.4 | 90.7 | 94.6 | 92.4 | 90.3 | 93.2 | 95.9 | 88.7 | 92.6 | 92.5 | 92.9 | 83.0 | 75.8 | 79.6 | 77.3 | |
| 8 | S. downei ATCC 33748 | 89.5 | 90.2 | 92.2 | 90.9 | 90.2 | 98.0 | 89.5 | 87.4 | 87.5 | 91.6 | 88.0 | 88.8 | 87.1 | 88.0 | 89.2 | 87.9 | 89.2 | 86.6 | 87.8 | 86.6 | 90.3 | 90.1 | 89.1 | 95.0 | 88.7 | 87.5 | 90.1 | 80.0 | 73.1 | 77.0 | 75.6 | |
| 9 | S. dysgalactiae ATCC 43078 | 86.9 | 92.2 | 85.6 | 85.0 | 83.0 | 84.3 | 92.8 | 85.6 | 93.4 | 90.0 | 91.9 | 89.6 | 86.2 | 93.4 | 87.8 | 93.7 | 92.5 | 93.7 | 92.9 | 98.6 | 88.6 | 92.2 | 92.4 | 87.0 | 91.3 | 95.0 | 92.1 | 81.6 | 75.7 | 78.6 | 76.9 | |
| 10 | S. equi ATCC 9528 | 88.9 | 92.2 | 85.0 | 86.9 | 83.0 | 84.3 | 92.2 | 86.3 | 90.2 | 89.2 | 90.8 | 88.4 | 86.6 | 90.3 | 87.9 | 90.8 | 90.9 | 90.5 | 90.4 | 92.8 | 88.6 | 90.3 | 90.1 | 86.6 | 90.3 | 90.3 | 90.1 | 80.2 | 74.2 | 77.5 | 75.6 | |
| 11 | S. ferus ATCC 33477 | 90.9 | 86.9 | 87.6 | 84.3 | 86.9 | 88.2 | 85.6 | 90.2 | 84.3 | 84.3 | 90.3 | 89.9 | 88.3 | 91.1 | 90.4 | 90.5 | 91.9 | 90.0 | 90.5 | 89.4 | 91.7 | 91.7 | 90.5 | 89.9 | 91.7 | 90.9 | 91.2 | 80.6 | 75.6 | 79.9 | 77.4 | |
| 12 | S. gordonii ATCC 10558 | 89.5 | 90.9 | 88.2 | 88.9 | 86.9 | 90.2 | 94.1 | 90.9 | 88.9 | 89.5 | 85.0 | 90.7 | 87.8 | 95.1 | 87.8 | 94.4 | 95.1 | 89.8 | 95.0 | 91.7 | 90.7 | 92.5 | 97.2 | 87.9 | 92.4 | 91.3 | 92.2 | 82.9 | 76.2 | 78.6 | 77.5 | |
| 13 | S. intermedius ATCC 27335 | 89.5 | 87.6 | 98.0 | 90.9 | 96.7 | 89.5 | 88.9 | 91.5 | 85.0 | 84.3 | 88.2 | 87.6 | 90.3 | 91.3 | 90.4 | 91.3 | 91.1 | 89.2 | 91.7 | 89.2 | 90.8 | 90.9 | 90.8 | 88.7 | 88.8 | 90.8 | 91.1 | 81.3 | 76.1 | 79.1 | 77.7 | |
| 14 | S. macacae ATCC 35911 | 88.9 | 87.6 | 90.2 | 89.5 | 92.2 | 87.6 | 87.6 | 88.2 | 83.7 | 85.6 | 84.3 | 87.6 | 90.2 | 86.7 | 90.1 | 86.3 | 88.6 | 86.9 | 86.7 | 88.5 | 90.8 | 87.6 | 87.0 | 88.3 | 87.1 | 86.5 | 87.8 | 79.0 | 73.9 | 77.3 | 75.3 | |
| 15 | S. mitis ATCC 49456 | 88.9 | 88.9 | 88.2 | 85.6 | 85.6 | 85.0 | 89.5 | 86.9 | 92.8 | 85.6 | 84.3 | 92.2 | 87.6 | 85.6 | 87.8 | 97.1 | 93.3 | 91.3 | 98.7 | 92.8 | 89.9 | 93.0 | 95.5 | 87.9 | 93.0 | 93.0 | 92.9 | 83.7 | 76.9 | 78.2 | 77.3 | |
| 16 | S. mutans ATCC 25175 | 85.0 | 85.0 | 90.9 | 93.5 | 88.9 | 88.2 | 84.3 | 88.2 | 81.7 | 83.0 | 84.3 | 84.3 | 90.2 | 88.2 | 83.7 | 86.9 | 88.7 | 88.0 | 86.9 | 87.3 | 91.2 | 88.3 | 87.8 | 89.5 | 87.5 | 87.3 | 88.2 | 79.6 | 75.7 | 79.4 | 76.7 | |
| 17 | S. oralis ATCC 35037 | 88.9 | 92.8 | 88.2 | 85.6 | 85.6 | 85.6 | 93.5 | 87.6 | 96.7 | 89.5 | 85.6 | 89.5 | 87.6 | 86.3 | 96.1 | 82.4 | 93.7 | 91.5 | 97.4 | 93.0 | 89.4 | 93.3 | 94.5 | 87.3 | 93.2 | 93.4 | 93.2 | 82.9 | 76.5 | 79.1 | 77.7 | |
| 18 | S. parasanguinis ATCC 15912 | 88.2 | 92.2 | 86.9 | 87.6 | 86.9 | 89.5 | 95.4 | 90.2 | 90.2 | 89.5 | 86.3 | 94.8 | 87.6 | 86.9 | 89.5 | 84.3 | 92.2 | 90.9 | 93.4 | 92.2 | 91.5 | 93.8 | 95.0 | 88.7 | 92.9 | 92.2 | 93.3 | 82.3 | 76.3 | 80.8 | 78.3 | |
| 19 | S. parauberis DSM 6631 | 83.7 | 91.5 | 82.4 | 82.4 | 82.4 | 81.0 | 90.9 | 82.4 | 96.7 | 87.6 | 82.4 | 86.3 | 83.0 | 84.3 | 90.2 | 80.4 | 93.5 | 88.2 | 91.1 | 93.6 | 88.4 | 90.4 | 89.9 | 86.1 | 89.9 | 92.6 | 90.1 | 80.7 | 76.2 | 78.8 | 76.3 | |
| 20 | S. pneumoniae ATCC 27336 | 90.2 | 89.5 | 89.5 | 86.3 | 86.9 | 85.6 | 90.2 | 87.6 | 93.5 | 86.3 | 85.6 | 92.8 | 88.9 | 86.9 | 98.7 | 83.0 | 96.1 | 90.2 | 90.9 | 92.2 | 89.8 | 92.6 | 95.4 | 87.5 | 93.0 | 92.9 | 92.5 | 83.6 | 77.0 | 78.6 | 77.5 | |
| 21 | S. pyogenes ATCC 19615 | 86.9 | 92.2 | 85.6 | 85.0 | 83.0 | 84.3 | 92.8 | 85.6 | 100.0 | 90.2 | 84.3 | 88.9 | 85.0 | 83.7 | 92.8 | 81.7 | 96.7 | 90.2 | 96.7 | 93.5 | 88.0 | 91.6 | 92.1 | 86.6 | 91.1 | 94.4 | 91.5 | 81.3 | 75.6 | 78.4 | 76.7 | |
| 22 | S. ratti ATCC 19645 | 90.2 | 88.2 | 93.5 | 94.1 | 91.5 | 89.5 | 88.9 | 89.5 | 85.0 | 86.3 | 85.6 | 90.9 | 91.5 | 93.5 | 86.3 | 90.2 | 86.3 | 88.2 | 82.4 | 87.6 | 85.0 | 90.7 | 90.8 | 91.1 | 89.6 | 88.3 | 89.9 | 82.8 | 74.6 | 78.6 | 76.3 | |
| 23 | S. salivarius ATCC 7073 | 88.2 | 92.2 | 87.6 | 88.2 | 87.6 | 86.3 | 89.5 | 86.9 | 86.9 | 85.6 | 83.0 | 88.9 | 86.9 | 87.6 | 87.6 | 83.7 | 90.2 | 92.8 | 86.9 | 87.6 | 86.9 | 88.2 | 92.4 | 90.1 | 93.2 | 92.1 | 99.1 | 81.3 | 76.1 | 79.0 | 77.9 | |
| 24 | S. sanguinis ATCC 10556 | 87.6 | 90.2 | 88.9 | 88.2 | 86.3 | 88.2 | 94.8 | 90.2 | 89.5 | 88.9 | 85.6 | 98.0 | 88.2 | 86.3 | 92.8 | 83.7 | 90.2 | 92.8 | 86.9 | 93.5 | 89.5 | 88.9 | 87.6 | 89.0 | 92.0 | 91.3 | 92.1 | 83.4 | 75.8 | 79.0 | 77.4 | |
| 25 | S. sobrinus ATCC 33478 | 89.5 | 87.6 | 94.1 | 90.9 | 92.2 | 92.2 | 85.6 | 94.1 | 83.0 | 85.6 | 86.9 | 86.9 | 92.8 | 92.2 | 85.0 | 89.5 | 85.6 | 86.3 | 81.0 | 86.3 | 83.0 | 91.5 | 85.6 | 86.3 | 88.4 | 87.1 | 90.3 | 80.9 | 74.1 | 77.3 | 75.7 | |
| 26 | S. suis ATCC 43765 | 89.5 | 90.2 | 84.3 | 83.7 | 83.0 | 83.0 | 88.9 | 85.0 | 89.5 | 87.6 | 86.3 | 86.9 | 83.7 | 83.7 | 88.2 | 79.7 | 92.2 | 89.5 | 88.9 | 88.2 | 89.5 | 84.3 | 88.2 | 86.3 | 83.7 | 91.5 | 93.2 | 81.5 | 75.7 | 77.9 | 77.9 | |
| 27 | S. uberis ATCC 19436 | 88.2 | 92.2 | 86.9 | 85.0 | 84.3 | 85.0 | 91.5 | 86.9 | 97.4 | 88.9 | 86.9 | 87.6 | 86.3 | 85.0 | 92.8 | 83.0 | 96.7 | 90.2 | 94.1 | 93.5 | 97.4 | 85.0 | 88.2 | 88.2 | 84.3 | 89.5 | 92.0 | 81.2 | 75.6 | 79.2 | 76.9 | |
| 28 | S. vestibularis ATCC 49124 | 88.2 | 90.2 | 86.3 | 85.6 | 86.3 | 85.6 | 88.9 | 86.3 | 86.9 | 85.6 | 82.4 | 88.2 | 85.6 | 88.2 | 87.6 | 82.4 | 90.2 | 92.2 | 86.3 | 87.6 | 86.9 | 86.3 | 97.4 | 86.9 | 85.6 | 87.6 | 88.2 | 81.5 | 76.0 | 79.0 | 77.8 | |
| 29 | L. lactis ATCC 19435 | 84.3 | 80.4 | 81.7 | 82.4 | 80.4 | 82.4 | 81.0 | 81.0 | 82.4 | 79.1 | 81.0 | 82.4 | 81.0 | 83.7 | 82.4 | 81.0 | 82.4 | 81.0 | 79.1 | 83.7 | 82.4 | 85.0 | 81.0 | 82.4 | 80.4 | 77.8 | 83.0 | 82.4 | 75.4 | 77.3 | 76.3 | |
| 30 | E. durans ATCC 19432c | 71.9 | 69.9 | 67.3 | 64.7 | 66.0 | 64.7 | 66.7 | 65.4 | 69.3 | 67.3 | 68.6 | 67.3 | 66.7 | 64.7 | 68.0 | 64.7 | 69.9 | 68.0 | 67.3 | 69.3 | 69.3 | 65.4 | 68.0 | 66.0 | 66.7 | 69.3 | 69.3 | 68.0 | 68.6 | 77.7 | 88.3 | |
| 31 | E. durans ATCC 19432 tufBc | 70.6 | 71.9 | 72.5 | 70.6 | 71.9 | 72.5 | 75.8 | 72.5 | 74.5 | 71.9 | 70.6 | 73.2 | 72.5 | 69.3 | 71.9 | 72.5 | 75.2 | 77.8 | 73.9 | 72.5 | 74.5 | 71.9 | 73.2 | 71.9 | 71.9 | 71.2 | 74.5 | 73.9 | 73.9 | 69.3 | 78.4 | |
| 32 | E. faecalis V853 | 76.5 | 74.5 | 69.3 | 68.6 | 69.9 | 70.6 | 70.6 | 71.2 | 71.9 | 71.9 | 73.2 | 70.6 | 68.6 | 69.9 | 69.9 | 68.0 | 71.9 | 71.9 | 71.2 | 70.6 | 71.9 | 67.3 | 73.9 | 69.9 | 68.6 | 74.5 | 72.5 | 73.9 | 71.9 | 86.9 | 74.5 | |
a
The streptococcal sequences were also compared with those obtained from E. durans and L. lactis. The numbers in the upper right triangle are the percentages of identity for a 761-bp segment from the tuf genes (positions 340 to 1100 of the complete gene of S. pneumoniae R6; accession number AE008504), while those in the lower left triangle represent the percentages of identity for the 153-bp sequences (positions 706 to 858 of the complete gene of S. pneumoniae R6; accession number AE008504) flanked by the two Streptococcus-specific PCR primers. The identity scores were obtained by use of the GAP program from the GCG Wisconsin package.
b
Species numbers are arbitrarily assigned to clarify species comparisons.
c
Two divergent copies of the tuf gene are found in E. durans (24).