Table 2. PCR and sequencing primers.
| Primer | Nucleotide position (nt) | Primer sequence | Orientation | Reference |
|---|---|---|---|---|
| 0001S48a | GGGGACAAGTTTGTACAAAAAAGCAGGCTTTAAAACAGCTCTGGGGTT | Forward | 38 | |
| AFP452-1446A | 1427–1446 | TGAACCACCTTGCCTGTAAC | Reverse | This study |
| AFP452-1888A | 1869–1888 | CACAACGGAATCAACCTCAG | Reverse | This study |
| AFP452-2522A | 2503–2522 | CTGGGATATGGGTGGAGTTG | Reverse | This study |
| AFP452-2584A | 2565–2584 | TGTTTGCAATGTGTCACCAG | Reverse | This study |
| 008 | 2411–2430 | GCRTGCAATGAYTTCTCWGT | Forward | 21 |
| 011 | 3389–3408 | GCICCIGAYTGITGICCRAA | Reverse | 21 |
| AFP452-3247S | 3247–3267 | CATCACTGACGAACGAACTGA | Forward | This study |
| AFP452-3959S | 3959–3978 | ACAGTGACTGCCACTCTAGC | Forward | This study |
| AFP452-4669S | 4669–4688 | AATGGCCGCATTGGAGGAGA | Forward | This study |
| AFP452-5298S | 5298–5317 | AGTTGTTCGCAGGATTCCAG | Forward | This study |
| AFP452-5919S | 5919–5938 | TTAATGATGAGCAAGGAGAG | Forward | This study |
| AFP452-6638S | 6638–6657 | GGATATGATGCCAGCTTGAG | Forward | This study |
| 7500Aa | GGGGACCACTTTGTACAAGAAAGCTGGG(T)24 | Reverse | 38 |
aThe primer pairs 0001S48/011 and 008/7500A are suggested for long distant PCR. The expected amplicons from these are 3.40 kb and 4.99 kb, respectively.