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. 2016 Aug 8;6:30784. doi: 10.1038/srep30784

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Copyright © 2016, The Author(s)

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PP cells and fecal pellets were collected from 3, 4, 6 and 8 week old IL-21eGFP mice and analysed. (a) Representative flow cytometry plots of GFP expression in gated CD4⁺ cells. (b) Representative flow cytometry plots of gated CD4⁺ cells show appearance of CXCR5⁺PD1⁺ Tfh cells in PP with age. (c) Percentage of CD4⁺ cells expressing GFP (solid line), and QPCR quantification of IL21 mRNA levels in PP (dotted line). (d) Percentage of CXCR5⁺PD1⁺ Tfh cells in PP CD4⁺ cells. (e) Percentage of PP Tfh cells expressing GFP. (f) Representative flow cytometry plots showing IgA⁺ and IgG1⁺ cells in gated PP CD19⁺ cells. (g) Quantification of the percentage of IgA⁺ and IgG1⁺ cells in PP CD19⁺ cells. (h) IgA levels in fecal extract supernatants as measured by ELISA. Data are expressed as mean ± s.e.m of 3–8 mice per group and are representative of two independent experiments.