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. 2016 Aug 8;6:31295. doi: 10.1038/srep31295

Figure 3. Comparison of EP and control peptides on VEGFR2 autophosphorylation.

Figure 3

(A) Dose-response of VEGFR2 phosphorylation by the selected EP peptides (EP3, EP5 and EP6). PAEC overexpressing VEGFR2 were treated with increasing concentrations (0.5, 5 and 50 ng/mL) of VEGF, EP3, EP5 and EP6 peptides (15 min at 37 °C). AcHx peptide was used as control. (B) Western blot quantification. Data in the graph report VEGFR2 phosphorylation increase respect to cells in serum deprivation condition (n = 3). (C) Comparison of the effect of EP6 and control peptides, tested alone and in combination, on VEGFR2 phosphorylation. PAEC-VEGFR2 were exposed to EP6 (50 ng/mL), AcHx (22 ng/mL), AcHn (26 ng/mL) and their combination for 15 min. Cell lysates were analyzed by immunoblotting using anti-phosphotyrosine antibody. Anti-VEGFR2 antibody was used as loading control. (western blot quantification expressed as fold increase respect to Ctr: AcHx 4.9 ± 1.3, AcHn 5.1 ± 0.95, combination 1.4 ± 0.89, EP6 5.4 ± 1.1).