PFGE and Southern hybridization of SmaI-digested genomic DNA and plasmid DNA from enterococcal isolates with a psoralen-biotin-labeled probe specific for prgB. (A and B) PFGE patterns of genomic DNA (A) and corresponding hybridizing bands (B). (C and D) PFGE profiles of plasmid DNA (C) and corresponding hybridizing bands (D). Lanes: 1, CIUM22; 2, CIUM34; 3, EFM26; 4, EFS12; 5, EGL19; 6, EFS20; 7, EFS27B; 8, EFS28B; 9, EFS30D; 10, EFS38; 11, OG1RF(pCF10); 12, OG1RF; M1, Low Range PFG Marker; M2, Marker II. The arrows indicate chromosomal bands (B, lanes 4, 5, and 6) or plasmid bands (B and D, lanes 7, 8, 9, and 11) hybridizing with the prgB probe.