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. 2004 Aug;42(8):3419–3427. doi: 10.1128/JCM.42.8.3419-3427.2004

FIG. 4.

FIG. 4.

Restriction analysis of PCR products obtained using primers internal to prgB and agg genes of clumping-positive (EFS-27b, EFS-30d, and EGL-19) and clumping-negative (EFS-12, EFS-20, and EFS-28b) clinical enterococcal isolates and of E. faecalis OG1RF(pCF10). (A) DraI digests from 427-bp amplicons (prgB). (B) EcoRI digests from 1,553-bp amplicons (agg). Lanes: 1, EFS-28b undigested; 2, EFS-28b digested; 3, EFS-30d undigested; 4, EFS-30d digested; 5, OG1RF(pCF10) undigested; 6, OG1RF(pCF10) digested; 7, EFS-20 undigested; 8, EFS-20 digested; 9, EFS-27b undigested; 10, EFS-27b digested; 11, EFS-12 undigested; 12, EFS-12 digested; 13, EGL-19 undigested; 14, EGL-19 digested; 15, Marker Gene Ruler 100-bp DNA ladder. The arrows indicate the sizes of fragments in base pairs.