Figure 6. hTAF4-TAFH governs AS events of different TFIID subunits and global differentiation and migratory potential of NC-derived cells.

(A) RT-PCR analysis of TBP, TAF1, TAF2, TAF6, TAF10 and TAF12 in response to TAFH RNAi in primary fibroblasts and cultured melanoma WM 266-4 and SkMel28 cells was performed by using transcript-specific primers. Respective ASVs that were sequence verified are denoted on the left and characterised in more detail in Figure S2. (B) High levels of proteins with intact hTAF4-TAFH (TAFH) are inherent to the stem and stem-like cells with low capacity to migrate, while abolished hTAF4-TAFH activity (∆TAFH protein isoforms) is characteristic to highly motile cells committed to differentiate. High levels of expression are shown in bold. Yellow colour indicates to the expression of TAF4 proteins in normal NC-derived cells (facial fibroblasts and melanocytes), whereas purple area relates to melanoma cells. Data of TAFH overexpression in fibroblasts are taken from⁴³. Scheme is adapted from⁷¹. iMel–induced melanocytes; iCSC-like cells—induced cancer stem cell-like cells.