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. 2016 Aug 8;6:30917. doi: 10.1038/srep30917

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Copyright © 2016, The Author(s)

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(a) Screening by PCR array of 84 mouse genes involved in the migration, adhesion and invasion processes (only the 66 genes amplified are displayed). A17 cells were treated for 48 h with sublethal doses of PRSE or left untreated. After RNA extraction and retrotranscription, the resulting cDNA was applied in the array and amplified. Data are reported as fold compared to control untreated A17 cells. Genes showing a regulation exceeding the 2-fold criteria were considered as differentially expressed. Data are reported as the mean ± standard deviation of three independent experiments. The transcripts of the following genes were not amplified: Cdh1, Cdh6, Cdh8, Cxcr2, Elane, Etva, Ewsr1, Fgfr4, Hgf, Il1b, Kiss1, Mmp13, Mmp7, Mycl, Rorb, Timp4, Tnfsf10 and Tshr. (b) Western blot analysis of total cell lysates obtained from PRSE-treated and untreated (n.t.) A17 cells.