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. 2016 Aug 8;6:31215. doi: 10.1038/srep31215

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Copyright © 2016, The Author(s)

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(a) μMED was validated by performing the enzyme amplification assay on-chip and comparing the results to flow cytometry on the assay’s microbeads. (b) We performed a serial dillution of cultured media from our cortical neuron in vitro model to measure the limit of detection. Device response was compared for N = 4 stretched, N = 4 not stretched, and N = 4 negative controls on both the μMED (c) device and on flow (N = 9 stretched, N = 11 not stretched, and N = 7 negative controls) (d), which showed qualitatively similar results. Error bars represent Standard Error of the Mean (SEM). *P < 0.05.