TABLE 2.
Correlation of multiplex PCR and conventional serotyping results for Listeria tested strains
| Species | Serovar | No. of strains | Multiplex PCR fragment amplification
|
Multiplex PCR serovar (sv) group classification | ||||
|---|---|---|---|---|---|---|---|---|
| lmo1118 (906 bp) | lmo0737 (691 bp) | ORF2110 (597 bp) | ORF2819 (471 bp) | prs (370 bp) | ||||
| L. monocytogenes | 1/2a | 40 | −a | + | − | − | + | L. monocytogenes sv 1/2a, 3a |
| 1/2b | 40 | − | − | − | + | + | L. monocytogenes sv 1/2b, 3b, 7 | |
| 1/2c | 40 | + | + | − | − | + | L. monocytogenes sv 1/2c, 3c | |
| 4b | 40 | − | − | + | + | + | L. monocytogenes sv 4b, 4d, 4e | |
| 3a | 5 | − | + | − | − | + | L. monocytogenes sv 1/2a, 3a | |
| 3b | 5 | − | − | − | + | + | L. monocytogenes sv 1/2b, 3b, 7 | |
| 3c | 5 | + | + | − | − | + | L. monocytogenes sv 1/2c, 3c | |
| 7 | 5 | − | − | − | + | + | L. monocytogenes sv 1/2b, 3b, 7 | |
| 4d | 5 | − | − | + | + | + | L. monocytogenes sv 4b, 4d, 4e | |
| 4e | 5 | − | − | + | + | + | L. monocytogenes sv 4b, 4d, 4e | |
| 4a | 5 | − | − | − | − | + | Listeria species | |
| 4c | 5 | − | − | − | − | + | Listeria species | |
| L. innocua | 6a | 5 | − | − | − | − | + | Listeria species |
| 6b | 5 | − | − | − | − | + | Listeria species | |
| L. ivanovii | 5 | 8 | − | − | − | − | + | Listeria species |
| L. seeligeri | 1/2b | 2 | − | − | − | − | + | Listeria species |
| L. welshimeri | 4c | 1 | − | − | − | − | + | Listeria species |
| 6a | 1 | − | − | − | − | + | Listeria species | |
a
The L. monocytogenes serovar 1/2a EGDe strain, in which the lmo1118 gene was first identified, was the only strain from the serovar group 1/2a tested to amplify the 906-bp lmo1118 fragment.