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. 2016 Aug 8;6:30941. doi: 10.1038/srep30941

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Copyright © 2016, The Author(s)

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(A) miR-370 expression following 5-azacitidine treatment of T24 cells. Results are mean ± SE ***p < 0.0005 (n = 3). (B,C) qRT-PCR analysis of DNMT1 (B) and IL-6 (C) mRNA expression in normal bladder cells (Normal) and a cancer cell line (T24; Cancer). Data are mean ± SE, **p < 0.0005, *** < 0.00001 (n = 3). (D) qRT-PCR analysis of DNMT1 mRNA expression in T24 cells after treatment with IL-6. (E–H) qRT-PCR analysis of IL-6 (E), DNMT1 (F), miR-370 (G) and SLD5 (I) expression in T24 cells 48 hours after transfection of IL-6-specific siRNA or control siRNA (ctl). Data are mean ± SE, *p < 0.05, **p < 0.005, ***p < 0.00001 (n = 3). (I) Western blotting for detection of SLD5 in cells as described in (E).