TABLE 4.
Interlaboratory agreement for caspofungin susceptibility data with 20 isolates of Aspergillus spp.a
| Test conditions | Endpoint | Visual readings
|
Spectrophotometer results
|
||
|---|---|---|---|---|---|
| Agreement range (%) | Excellent agreement (%) | Agreement range (%) | Excellent agreement (%) | ||
| RPMI, 24 h | MIC0 or IC90 | 73.5-100.0 | 75.0 | 34.4-93.1 | 5.0 |
| MIC2 or IC50 | 46.8-91.2 | 5.0 | 27.5-86.2 | 0.0 | |
| MEC | 47.0-90.6 | 5.3 | |||
| MEC (modified)b | 91.1-100.0 | 100.0 | |||
| RPMI, 48 h | MIC0 or IC90 | 38.2-79.4 | 0.0 | 48.0-96.6 | 20.0 |
| MIC2 or IC50 | 50.0-96.4 | 10.0 | 20.0-72.0 | 0.0 | |
| RP-G, 24 h | MIC0 or IC90 | 82.3-100.0 | 70.0 | 37.9-93.1 | 5.0 |
| MIC2 or IC50 | 37.5-96.9 | 15.0 | 24.1-82.8 | 0.0 | |
| MEC | 50.0-96.9 | 0.0 | |||
| MEC (modified) | 85.2-100.0 | 75.0 | |||
| RP-G, 48 h | MIC0 or IC90 | 52.9-88.2 | 0.0 | 52.1-95.7 | 55.0 |
| MIC2 or IC50 | 57.6-100.0 | 30.0 | 24.0-80.0 | 0.0 | |
| AM3, 24 h | MIC0 or IC90 | 57.6-100.0 | 70.0 | 41.3-93.1 | 35.0 |
| MIC2 or IC50 | 32.3-94.1 | 10.0 | 31.0-89.7 | 0.0 | |
| MEC | 60.0-96.7 | 15.0 | |||
| MEC (modified) | 81.2-100.0 | 85.0 | |||
| AM3, 48 h | MIC0 or IC90 | 26.4-91.2 | 10.0 | 44.8-93.1 | 5.0 |
| MIC2 or IC50 | 69.2-100.0 | 65.0 | 37.9-89. | 0.0 | |
a
High levels of agreement reflect predominantly off-scale readings. Agreement range, percentage of results within excellent agreement range across all 30 isolates; excellent agreement, percentage of isolates with ≥90% interlaboratory agreement.
b
MEC (modified), data from laboratories E, H, and J were excluded from analysis.