TABLE 1.
Comparison of results obtained by DNA sequencing with those obtained by discriminatory real-time PCR
| Time pointa | Variantb | No. of concordant samples | No. of samples showing mix with PCR but not sequencing | No. of discordant samples | VLc mean (range) log copies per ml |
|---|---|---|---|---|---|
| A | Wild type | 38 | 0 | 0 | 7.95 (3.86-8.79) |
| B | M204V | 9 | 9 | 3 | 7.34 (4.59-8.88) |
| M204I | 0 | 2 | 12 | 7.92 (3.67-7.81) | |
| Wild type | 10 | 2 | 0 | 6.63 (4.08-8.28) | |
| Total | 19 | 13 | 15 | ||
| C | M204I | 9 | 1 | 0 | 8.08 (4.79-9.00) |
a
Time points are the following: A, pretherapy; B, posttherapy, prior to addition of the second M204I primer; C, posttherapy, following addition of the second M204I primer.
b
As defined by direct sequencing.
c
VL, HBV viral load.