FIG. 2.
Dilution series of HPV16 (106 to 10−1 copies). Comparison of sensitivity between paraffin- and non-paraffin-embedded material performed by conventional PCR and real-time PCR visualized on gel. Rows: A, conventional PCR, paraffin-embedded material (DNA from archival HPV-negative cervical cancer tissue sample); B, conventional PCR, non-paraffin-embedded material (DNA from HeLa-cells); C, real-time PCR, paraffin-embedded material (DNA from archival HPV-negative cervical cancer tissue sample); D, real-time PCR, non-paraffin-embedded material (DNA from HeLa cells).