TABLE 1.

Primers used for PCR and for sequencing of 16S and 23S rRNA genes of B. cereus group bacteria^a

Name	Sequence	Location
P1	5′-GTT TGA TCC TGG CTC AG	11-27 (16S rRNA)
P10	5′-CCA GTC TTA TGG GCA GGT TAC	136-116 (16S rRNA)
P11	5′-TCC ATA AGT GAC AGC CGA AGC	226-206 (16S rRNA)
P5	5′-CTA CGG GAG GCA GCA GTG GG	340-360 (16S rRNA)
P3	5′-GWA TTA CCG CGG CKG CTG	535-517 (16S rRNA)
P2	5′-GGA TTA GAT ACC CTG GTA GT	784-803 (16S rRNA)
P6	5′-CCG TCA ATT CCT TTR AGT TT	926-907 (16S rRNA)
P8	5′-TTC GGG AGC AGA GTG ACA GGT	1029-1049 (16S rRNA)
P9	5′-TAC ACA CCG CCC GTC ACA CCA	1392-1412 (16S rRNA)
P4	5′-RGT GAG CTR TTA CGC	1513-1492 (16S rRNA)
Pr1	5′-CCG AAT GGG GVA ACC C	114-129 (23S rRNA)
Pr13	5′-CCG TTT CGC TCG CCG CTA CTC	262-242 (23S rRNA)
PB1	5′-TAG TGA TCG ATA GTG AAC CAG	485-505 (23S rRNA)
Pr2	5′-CAT TMT ACA AAA GGY ACG C	621-603 (23S rRNA)
Pr3	5′-GCG TRC CTT TTG TAK AAT G	603-621 (23S rRNA)
PB2	5′-TAG TGA TCG ATA GTG AAC CAG	755-736 (23S rRNA)
PB3	5′-TAG TGA TCG ATA GTG AAC CAG	969-990 (23S rRNA)
Pr4	5′-RGT GAG CTR TTA CGC	1151-1137 (23S rRNA)
Pr5	5′-WGC GTA AYA GCT CAC	1136-1150 (23S rRNA)
PB4	5′-CAT ACC GGC ATT CTC ACT TC	1308-1289 (23S rRNA)
PB5	5′-ACA GGC GTA GGC GAT GGA C	1408-1426 (23S rRNA)
PB8	5′-AAC CTT TGG GCG CCT CC	1679-1661 (23S rRNA)
Pr6	5′-CYA CCT GTG WCG GTT T	1673-1659 (23S rRNA)
Pr7	5′-AAA CCG WCA CAG GTR G	1659-1673 (23S rRNA)
Pr8	5′-CAY GGG GTC TTT RCG TC	2092-2076 (23S rRNA)
Pr9	5′-GAC GYA AAG ACC CCR TG	2076-2092 (23S rRNA)
Pr10	5′-GAG YCG ACA TCG AGG	2535-2521 (23S rRNA)
Pr11	5′-CCT CGA TGT CGR CTC	2521-2535 (23S rRNA)
Pr12	5′-GYT TAG ATG CYT TC	2783-2770 (23S rRNA)
R1	5′-GGC GGC GTC CTA CTC TCA C	112-95 (5S rRNA)

^aPrimers P1 to P4, Pr1 to Pr7, and R1 were used for DNA amplification. All other primers were utilized for sequencing. Primers P8, P9, P10, and P11 were selected de novo; other primers were described earlier (for details, see reference 32).