TABLE 3.
Classification of bacteria in the B. cereus group according to 23S rRNA sequences
| Subgroup name | Subgroup-specific signature(s) (position)a | Start and end of sequence | Organism | GenBank accession no. | Position(s) of strain-specific variation(s) |
|---|---|---|---|---|---|
| Anthracis | Consensusa | 1-2922 | B. anthracis Sterneb | AF267877 | —k |
| 1-2922 | B. anthracis Delta Ames-1b | AF267876 | — | ||
| 1-2922 | B. anthracis Ames ANRb | AF267734 | — | ||
| 1-2922 | B. anthracis Ames (TIGR)c | AE017024 to -26, AE017039 | — | ||
| 15-2943 | B. anthracis Sterned | S43426 | T/C(491), del CG(1413, 1414), T/C(2651) | ||
| Cereus A | Y/C(594)j | 1-2923 | B. cereus NCTC11143d | S43429 | T/C(2651) |
| G/A(1559) | 1-2923 | B. cereus ATCC 10987c | AE017264 to -66 | — | |
| Insertion G(1218-1219) | AE017280 | ||||
| 1-527 | B. cereus WSBC10030e | Z84589 | — | ||
| B. cereus HER1414f | ? | ||||
| 1-2923 | B. thuringiensis B8b | AF267880 | — | ||
| Cereus B | Y/C(594) | 1-2922 | B. cereus NCTC9620b | AF267878 | — |
| G/A(1559) | 1-2922 | B. cereus Tb | AF267879 | G/R(1559) | |
| T/A(2153) | 1-2787 | B. cereus DSM31g | X94448 | T/C(1275) | |
| 24-2789 | B. cereus LMG6923g | AJ310096 | — | ||
| 1-2922 | B. cereus ATCC 14579c,g | AF016998 to AF017000, AF017013 | — | ||
| 1-2922 | B. cereus BGSC 6A5c | AY224379 to AY224388 | — | ||
| 18-2897 | B. cereus Tim-r01 | AB050631 | ins G(1218-1219), G/T(1268), G/A(1557), ins T(1781-1782), T/A(1938) | ||
| 1-527 | B. thuringiensis WS2617e | Z84594 | — | ||
| 1-527 | B. thuringiensis WS2614e | Z84593 | — | ||
| Thuringiensis A | T/A(2153)Insertion G(1218-1219) | B. thuringiensis strs. 4R1,h 4D1,h 4F1,h 4T1,h 4W1,h 4S2,h and 4J4h | ? | ||
| Thuringiensis B | Y/T(594) | 1-2922 | B. thuringiensis 4Q281b | AF267881 | G/R(1559) |
| T/C(157) | 24-2789 | B. thuringiensis LMG7138i | AJ310738 | G/R(546) | |
| G/A(921), A/G(1020), C/T(1037), G/A(1209), A/G(1251), T/C(1283)C/T(132), A/T(174), G/T(1250) T/A(2153) | 1-2784 | B. thuringiensis DSM2046i | X89895 | C/T(57), T/G(413), ins AATA(479-480), del GG(541-542), G/A(646), C/G(670), G/A(1953), G/A(2055), ins AGT(2556-2557), del G(2573) | |
| B. thuringiensis 4A1,h 4A7,h 4Q1,h 4Q2,h and 4M1h | ? | ||||
| 1-2922 | B. medusa ATCC 25621b | AF267885 | — | ||
| 20-2790 | B. cereus ATCC 14893 | AJ310099 | CA/TC(265,266), T/C(358), G/A(646), C/T(655), G/A(663), C/G(1816), G/C(1849) | ||
| 24-2799 | B. cereus biovar toyoi CNCM 1-1012/NCIB 40112 | AJ310101 | CA/TC(265,266), G/A(646), C/T(655), G/A(663), C/G(1816), G/C(1849) | ||
| Mycoides A | Y/T(594) | 1-527 | B. mycoides DSM2048T | Z84592 | — |
| T/C(157) | 1-527 | B. mycoides MWS5303-1-4 | Z84591 | — | |
| G/A(921), A/G(1020), C/T(1037), G/A(1209), A/G(1251), T/C(1283) | 23-2789 | B. mycoides DSM2048 | AJ310097 | CA/AY(375, 376), T/C(1112), T/C(2651) | |
| CA/TC(265,266), GT/AC(364,365), C/G(1816), G/C(1849) | 1-527 | B. cereus WSBC10206 | Z84590 | — | |
| C/T(132), A/T(174), G/T(1250) T/A(2153) | |||||
| C/T(375) | |||||
| Mycoides B | Y/T(594) | 1-2922 | B. mycoides ATCC 6462mb | AF267884 | — |
| T/C(157) | 1-2922 | B. mycoides ATCC 10206b | AF267883 | — | |
| G/A(921), A/G(1020), C/T(1037), G/A(1209), A/G(1251), T/C(1283) | |||||
| CA/TC(265,266), GT/AC(364,365), C/G(1816), G/C(1849) | |||||
| GA/AG(346,347), TC/CT(358,359), C/A(482), C/T(672), A/T(1219), G/T(1268), A/G(2159) |
For more details, see Fig. 2.
23S rDNA sequenced in this work.
From sequences of whole genome; data represent average sequence of all available 23S rRNA genes.
Need to be reexamined (see Results).
Assigned to this subgroup in accordance with the 16S rRNA sequence (see Table 2).
Not sequenced; identified through hybridization analysis (see Results).
These three strains are identical and correspond to B. cereus IAM12605, B. cereus NCDO1771, B. cereus ATCC 11778, and B. cereus ATCC 14579; see 16S rRNA sequences of these strains in Table 2.
Not sequenced; subgroup-specific signatures A/G(77), T/C(90), and T/A(92) were identified by hybridization with subgroup-specific probes (S. Bavykin and J. Jackman, unpublished).
These two strains are identical and correspond to B. thuringiensis IAM12077, B. thuringiensis NCIMB9134, B. thuringiensis ATCC 10792, and B. thuringiensis BT3; see 16S rRNA sequences of these strains in Table 2. Y = C or T; R = G or A.
See footnote q of Table 2.
See footnote s of Table 2.