FIGURE 3.

Tobacco leaf infiltration assay demonstrates HR induction in response to direct physical interaction between Pi54 and candidate AvrPi54 proteins. Line diagrams represent Pi54 expression module (A) and AvrPi54 expression module (B) used for agroinfiltration. HR symptoms were observed when Pi54 and AvrPi54 expression modules were co-infiltrated in tobacco leaf (C). Neither AvrPi54 expression module nor Pi54 expression module produced HR when infiltrated individually (D,E, respectively). Empty pCAMBIA vector (negative control) also did not induce HR upon infiltration (F). The sites of agroinfiltrations in the tobacco leaves are marked by white dashed circles.