TABLE 1.
Characteristics of ESBL-producing Enterobacteriaceae strains isolated in a French university hospital in 2002
| Species (total no. of isolates) | Total no. (%) of ESBL-producing isolates | PFGE profilea | Specimen(s)b | Unit or ward (no.) | β-Lactamase contentc | Antibiotyped |
|---|---|---|---|---|---|---|
| E. aerogenes (162) | 29 (36.3) | EAIA (9) | Pus (5), urine (3), respiratory tract (1) | Medicine (4), ICU (2), surgery (2), geriatric | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL (6) |
| KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL (3) | ||||||
| EAIB (6) | Pus (3), urine (3) | Medicine (5), geriatric | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL (5) | ||
| KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL (1) | ||||||
| EAIC (4) | Urine (2), cutaneous (1), respiratory tract (1) | ICU (4) | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL (1) | ||
| EAIE (4) | Urine (3), pus (1) | Medicine, surgery, geriatric, recovery | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EAIG (3) | Urine (2), pus (1) | Medicine, geriatric, recovery | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EAIF | Pus | Surgery | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EAID | Urine | Recovery | TEM-24, AmpC | K-NAL, OFX, NOR, CIP, PEF-TET CHL | ||
| EAIH | Urine | Geriatric | TEM-24, AmpC | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| K. pneumoniae (182) | 15 (18.8) | KPI | Urine | Recovery | TEM-24, TEM-1 | KTNt(A)-NAL-SXT TET CHL |
| KPII | Urine | Medicine | TEM-3 | KTNt(A)-NAL-SXT TET CHL | ||
| KPIIIA (8) | Urine (5), cutaneous (1), pus (1), respiratory tract (1) | ICU (4), geriatric (2), surgery, recovery | TEM-3, TEM-1, SHV-1 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| KPIIIC (2) | Pus, respiratory tract | Surgery, medicine | TEM-3, TEM-1 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| KPIIIB | Urine | Medicine | TEM-3 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| KPIIID | Urine | Medicine | TEM-3, TEM-1, SHV-1 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| KPIIIE | Urine | Recovery | TEM-3, TEM-24 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| E. coli (1,827) | 13 (16.2) | ECIA (2) | Pus, urine | Medicine, geriatric | TEM-24, TEM-1 | KTNt(A)-NAL, OFX, NOR, PEF-SXT TET CHL |
| ECIIA (2) | Pus, urine | Medicine, surgery | CTX-M-3, TEM-1 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EC1 | Respiratory tract | Medicine | TEM-24 | KTNt(A)-NAL-SXT TET CHL | ||
| EC2 | Urine | Medicine | CTX-M-15, TEM-1/OXA-1 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EC3 | Urine | Medicine | CTX-M-15, OXA-1 | KTGNt(A)-NAL, OFX, NOR, PEF-SXT TET CHL | ||
| EC4 | Urine | Medicine | CTX-M-15, OXA-1 | KTGNt(A)-NAL, OFX, NOR, PEF-SXT TET CHL | ||
| EC5 | Pus | Surgery | CTX-M-14, TEM-1 | K(A)-NAL-SXT TET CHL | ||
| EC6 | Cutaneous | ICU | CTX-M-14, TEM-1 | K(A)-NAL-SXT TET CHL | ||
| EC7 | Venereal | ICU | CTX-M-15, TEM-1/OXA-1 | KTGNt(A)-NAL, OFX, NOR, PEF-TET CHL | ||
| EC8 | Urine | Geriatric | TEM-24 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| EC9 | Urine | Surgery | TEM-3 | KTNt(A)-NAL-TET CHL | ||
| C. koseri (59) | 12 (15) | CKIA (4) | Urine | Medicine (2), geriatric, ICU | TEM-24 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL |
| CKID (4) | Urine | Geriatric (2), medicine (2) | TEM-3 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| CKIE (2) | Urine | Recovery, ICU | TEM-3 | K(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| CKIB (1) | Urine | Surgery | TEM-3 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| CKIC (1) | Pus | Medicine | TEM-3 | K(A)-NAL, OFX, NOR, PEF-SXT TET CHL | ||
| P. mirabilis (258) | 6 (7.5) | PM1 | Urine | Medicine | TEM-3 | K(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL |
| PM2 | Urine | Medicine | TEM-3, TEM-1 | K(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| PM3 | Urine | ICU | TEM-3 | K(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| PM4 | Urine | Geriatric | TEM-3 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| PM5 | Cutaneous | Geriatric | TEM-3 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| PM6 | Cutaneous | Geriatric | TEM-3 | KTNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| P. stuartii (53) | 4 (5) | PS1 | Urine | Geriatric | TEM-24 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL |
| PS2 | Pus | Recovery | TEM-24 | KTGNt(A)-NAL, OFX, NOR, PEF-SXT TET CHL | ||
| PS3 | Urine | ICU | TEM-24 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-SXT TET CHL | ||
| PS4 | Urine | Recovery | TEM-24 | KTGNt(A)-NAL, OFX, NOR, CIP, PEF-TET CHL | ||
| K. oxytoca (82) | 1 (1.2) | KO1 | Urine | Medicine | TEM-3 | KTNt(A)-NAL-SXT TET CHL |
a
PFGE profiles were generated after restriction digestion of chromosomal DNA with the restriction enzyme XbaI.
b
The number of isolates is given in parentheses if more than one isolate was recovered.
c
AmpC and SHV-1, species-specific cephalosporinase and SHV-1 like chromosomal penicillinase, respectively.
d
Resistance to different antimicrobial agents. Abbreviations: T, tobramycin; A, amikacin; G, gentamicin; K, kanamycin; Nt, netilmicin; SXT, cotrimoxazole; NAL, nalidixic acid; OFX, ofloxacin; NOR, norfloxacin; CIP, ciprofloxacin; IPM, imipenem; TET, tetracycline; CHL, chloramphenicol. Parentheses indicate a low level of resistance.