TABLE 1.

Isolates used in this study and their expected toxin genotype

Isolate	Expected toxin genotype	Reference or source	Obtained toxin genotype in the present study bya:
			Unmodified method	Modified method
R5371/00	sea, seg, seh, sei, and tsst	FSML,b9	sea, seg, and seh	sea, seg, seh, sei, and tsst
R5460/00	seb, seg, seh, sei, and tsst	FSML, 9	seg and seh	seb, seg, seh, sei, and tsst
R5010/00	sed, seg, sei, and sej	FSML, 9	sed, seg, sei, and sej	sed, seg, sei, and sej
R4774/00	None	FSML, 9	None	None
R4571/00	sec and tsst	FSML, 9	sec	secc and tsst
R4071/00	seb	FSML, 9	None	seb
R2102/00	sec, seg, and sei	FSML, 9	seg and sei	sec,cseg, and sei
R963/00	sed, seg, sei, and sej	FSML, 9	sed, seg, sei, and sej	sed, seg, sei, and sej
FRI472	sed, seg, sei, and sej	12	sed, seg, sei, and sej	sed, seg, sei, and sej
FRI913	sea, sec, see, and tsst	12	sea, sec, and see	sea, sec,csee, and tsst
FRI572	seg and sei	12	seg and sei	seg and sei
FRI445	seg and sei	12	seg and sei	seg and sei
3169	secbovine, sed, sej, and tsst	12	sec, sed, and sej	secbovine, sed, sej, and tsst

^aOur results using MJ research PTC 225 and Perkin Elmer 9700 thermocyclers.

^bFSML, Jim McLauchlin, Food Safety Microbiology Laboratory, Public Health Laboratory Service, London, United Kingdom.

^cThis isolate generates both the seb-sec and the sec amplification products and should therefore be analyzed further with unique primers for seb to ensure that there is no seb hidden in the seb-sec product.