TABLE 3.
Primers and probes used for identification of Aspergillus spp. by PCR-EIA
| Primer or probe | Nucleotide sequence (5′ to 3′)a | Chemistry and location |
|---|---|---|
| Primers | ||
| ITS1 | TCC GTA GGT GAA CCT GCG G | 18S rDNA universal fungal 5′ primer |
| ITS3 | GCA TCG ATG AAG AAC GCA GC | 5.8S rDNA universal fungal 5′ primer |
| ITS4 | TCC TCC GCT TAT TGA TAT GC | 28S rDNA universal fungal 3′ primer |
| Probes | ||
| B58 | GAA TCA TCG A(AG)T CTT TGA ACG | 5′-End-labeled biotin probe; 5.8S region of filamentous fungi |
| AFLA | GAA CGC AAA TCA ATC TTT | 5′-End-labeled digoxigenin probe; ITS2 region of A. flavus |
| AFUM | CCG ACA CCC ATC TTT ATT | 5′-End-labeled digoxigenin probe; ITS2 region of A. fumigatus |
| ANID | GGC GTC TCC AAC CTT ATC | 5′-End-labeled digoxigenin probe; ITS2 region of A. nidulans |
| ANIG | GAC GTT ATC CAA CCA TTT | 5′-End-labeled digoxigenin probe; ITS2 region of A. niger |
| ATER | GCA TTT ATT TGC AAC TTG | 5′-End-labeled digoxigenin probe; ITS2 region of A. terreus |
| AUST | CTT TTA TTT TAC CAG GTT | 5′-End-labeled digoxigenin probe; ITS2 region of A. ustus |
| AVER | CTC CAA CCA TTT TCT TCA | 5′-End-labeled digoxigenin probe; ITS2 region of A. versicolor |
| ASPEN-G | CCT CGA GCG TAT GGG GCT | 5′-End-labeled digoxigenin probe; ITS2 region of Aspergillus and Penicillium spp. |
a
Patents have been issued or are pending for all Aspergillus species probes.