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. Author manuscript; available in PMC: 2016 Aug 8.
Published in final edited form as: Nat Neurosci. 2012 Jun 17;15(7):979–987. doi: 10.1038/nn.3135

Figure 1. Drosophila Crk, dCed-12, and Rac1 are required for glial clearance of severed axons from the CNS.

Figure 1

(a) OR85e-expressing ORNs were labeled with mCD8::GFP in control (w;OR85e-mCD8::GFP/+; repo-Gal4/+) and glial RNAi for crkRNAi (w;OR85e-mCD8::GFP/UAS-crk19061RNAi; repo-Gal4/+), dced-12RNAi (w;OR85e-mCD8::GFP/+; repo-Gal4/UAS-dced-1210455RNAi), and rac1RNAi (w, UAS-rac149247RNAi/+ ;OR85e-mCD8::GFP/+; repo-Gal4/+). Maxillary palps were bilaterally ablated and clearance of axonal debris from the CNS was assayed with anti-GFP antibody stains (green) 1, 5, 10, 15, and 30 days after injury.

(b) Normalized quantification of data to control from (a) Error bars represent s.e.m.; n>10 for all.