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. 2016 Aug 8;7:1225. doi: 10.3389/fmicb.2016.01225

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Copyright © 2016 Léonard, Bouarab Chibane, Ouled Bouhedda, Degraeve and Oulahal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dual parameter FCM dot plots. (I). Dual parameters with two discriminated dyes. I.1. Four different behaviors detected: quadrant A1, FL1−/FL2+; quadrant A2 FL1+/FL2+; quadrant A3, FL1−/FL2−; quadrant A4 FL1+/FL2−. I.2. Dots blots of Lactobacillus rhamnosus GG to assess the effects of different pressure (100,400,600 MPa) and thermal (95°C)treatment on esterase activity (cFDA) and membrane integrity (P1): quadrant A1, cF−/P1+; quadrant A2, cF+/P1+;quadrant A3, cF−/P1−; quadrant A4, cF+/P1− (Ananta et al., 2004). (II). Dual parameter dot plots with one discriminated dye. II. 1 Two different behaviors detected: initial state/Final state. II. 2.Syto9-CTC stained Arcobacter cryaeophilus LMG 10829 with resveratrol at concentration of 0 (control), 1xMIC and 4xMIC: initial state: Syto9+/CTC; Final state: Syto9+/CTC−(Ferreira et al., 2014).