Figure 2.

Lipopolysaccharide (LPS) upregulated the expression of transient receptor potential canonical 3 (TRPC3) and 4 (TRPC4) channels in rat pulmonary arteries and proliferation of pulmonary arterial smooth muscle cells (PASMCs). The expression levels of TRPC1/3/4/6 messenger RNA (mRNA) in cultured pulmonary arteries (A) and PASMCs (B) are shown. β-actin was used for normalization of mRNA. (C) Protein expression levels of TRPC3 and TRPC4. GAPDH was used as an internal control. * P<0.05; ** P<0.01; *** P<0.001; n=6 (mRNA) and n=3 (protein) in each group.