Table 1.
PCR primers for rat genes studied.
| Gene | Primer sequences (5′-3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
| TRPC1 | GGATTATTGGGATGATTTGGT GTGAGCCACCACTTTGAGG |
55 | 143 |
| TRPC3 | ACATCACCGTTATTGACTACCC GCACTCAGACCACATCATCC |
55 | 113 |
| TRPC4 | ACCATCGTGGAGTGGATGA TGTCGCCAGATACAAGGAGT |
55 | 147 |
| TRPC5 | CAACTGTCGTGGAATGGATG CCAGGTAGAGGGAGTTCATTG |
55 | 144 |
| TRPC6 | TCCGAATCTCAGCCGTTT ATGGTCTGCTGCCGTAAAC |
55 | 129 |
| TRPC7 | CGCCTACCTGTCCCTATCC CACGCCCACCACAAAGTC |
55 | 148 |
| β-actin | TGAACCCTAAGGCCAACC AGAGGCATACAGGGACAACA |
55 | 107 |