Table 1.

Primers for genomic PCR, cloning and sub-cloning.

Primer name*	Sequence	Target gene
[Genomic PCR]
HPT-F1	5′-ATTCCGGAAGTGCTTGACAT-3′	HPT (T-DNA)
HPT-R3	5′-CAGTCCTCGGAAAGCATCAG-3′
PDFR-F1	5′-TGGCGTTCCTTAAACAGCTT-3′	TfDFR promoter
PDFR-R2	5′-TGAACACCGTTGCTTTTTGA-3′
35S-F1	5′-CGCACAATCCCACTATCCTT-3′	35 S promoter
PhAN2-R1	5′-GGTCCATGCACCTTTCCTTA-3′	PhAN2
PhPH4-R1	5′-TGATGATGATGGGGTTCTCA-3′	PhPH4
InItNHX1-R1	5′-CCGAATTTTGGAGCAAAGAA-3′	InNHX1, ItNHX1
InNHX2-R1	5′-AGTGTCCCAACGTCAACTCC-3′	InNHX2
[Cloning]
AN2-UP-F3	5′-CTTTCTTTGTCCTTTAGTTGTCAGTTGCAGTGAG-3′	PhAN2
AN2-UP-R1	5′-AATTTCAAATGTCCAACGATTTCAACTGTAGTGTTT-3′
PH4-UP-F1	5′-CTCCCCTCCCAAATTTAGTACTATGACTTTATC-3′	PhPH4
PH4-UP-R3	5′-AACCCTCACATACATATATATATAGCTACAACATCA-3′
InNHX1-UP-F1	5′-CCCCACATCTCACCTTTCAAGTGATTTGTATGTTTT-3′	ItNHX1
InNHX1-UP-R1	5′-TGAGAAGACTTTCACCCAGCCCATTTCATAAGAG-3′
[Subcloning]
AN2-F1-SmaI	5′-AATAATCCCGGGATGAGTACTTCTAATGCATCAACATCAGGA-3′	PhAN2
AN2-R1-SmaI	5′-AATAATCCCGGGACTAACTAAATCCCATATGTCATCAATATCAACTG-3′
PhPH4-F1-SmaI	5′-AATAATCCCGGGATGAGAACCCCATCATCATCATCAACAA-3′	PhPH4
PhPH4-R1-SmaI	5′-AATAATCCCGGGACTGGGATTATATTGATCAGATGAAGGTGTGGA-3′
InNHX1-F1-SmaI	5′-AATAATCCCGGGATGGCGTTCGGGTTGTCTTCTT-3′	InNHX1
InNHX1-R1-SalI	5′-AATAATGTCGACTCATCTAGGGCTCTGCTCAACTGG-3′
InNHX2-F1-DraI	5′-AATAATTTTAAAATGGGAGTTGACGTTGGGACAC-3′	InNHX2
InNHX2-R1-SalI	5′-AATAATGTCGACTCACTGCCATTGTGGTTCATTCTG-3′
ItNHX1-F1-SmaI	5′-AATAATCCCGGGATGGCGTTCGGATTGTCTTCTTT-3′	ItNHX1
ItNHX1-R1-SalI	5′-AATAATGTCGACTCATCTAGGGCTCTGCTCAGCTG-3′
HSPt-F-SacI	5′-AAAGAGCTCATATGAAGATGAAGATGAAA-3′	HSP terminator
HSPt-R-EcoRI	5′-AAAGAATTCCTTATCTTTAATCATATTCC-3′

^*‘F’ represents forward primer and ‘R’ represents reverse primer.