Table 3.
Behavioural Analysis Before, During and After Noise Stress.
Before stress (10 min) |
During stress (10 min) |
After stress (10 min) |
|||||||
---|---|---|---|---|---|---|---|---|---|
Vehicle | 40% corticosterone | 100% corticosterone | Vehicle | 40% corticosterone | 100% corticosterone | Vehicle | 40% corticosterone | 100% corticosterone | |
Sitting (% time) | 90.6 ± 4.1 | 91.6 ± 2.2 | 97.7 ± 1.8 | 41.9 ± 8.3 | 71.4 ± 8* | 43.3 ± 6.3 | 58.3 ± 7.0 | 77.7 ± 9.7 | 83.8 ± 5.6 |
Total activity (% time) | 9.4 ± 4.1 | 8.4 ± 2.2 | 2.3 ± 1.8 | 58.1 ± 8.3 | 28.6 ± 9* | 56.7 ± 6.3 | 41.7 ± 7.0 | 22.2 ± 9.7 | 16.2 ± 5.6 |
Locomotion (% time) | 1.0 ± 0.5 | 1.1 ± 0.5 | 0.4 ± 0.3 | 10.9 ± 2.4 | 3.8 ± 1.2* | 7.1 ± 1.5 | 2.3 ± 0.7 | 0.7 ± 0.5 | 0.9 ± 0.4 |
Risk assessment (% time) | 0.5 ± 0.3 | 0.4 ± 0.3 | 0.4 ± 0.4 | 27.9 ± 7.4 | 12 ± 3.1* | 42 ± 6.0* | 7.3 ± 3.4 | 10.0 ± 5.7 | 6.3 ± 2.9 |
Face washing (% time) | 1.7 ± 1.1 | 1.9 ± 0.7 | 0.2 ± 0.2 | 17 ± 2.6 | 3.2 ± 1.7** | 6.6 ± 1.6* | 17.6 ± 4.1 | 4.8 ± 1.8** | 4.0 ± 1.9** |
Body grooming (% time) | 6.2 ± 3.0 | 3.8 ± 1.1 | 1.3 ± 1.0 | 6.0 ± 2.2 | 0.7 ± 0.7* | 1.0 ± 0.4* | 8.2 ± 2.0 | 5.2 ± 3.0 | 2.1 ± 1.3 |
Rearing (events) | 0 ± 0 | 0 ± 0 | 0.6 ± 0.6 | 7.5 ± 2.1 | 3.7 ± 1.8 | 10.3 ± 2.4 | 0.5 ± 0.3 | 0.4 ± 0.3 | 0.3 ± 0.3 |
Digging (events) | 0.3 ± 0.3 | 1.3 ± 1.0 | 0 ± 0 | 0.2 ± 0.2 | 2.0 ± 1.0 | 7.5 ± 2.1 | 4.5 ± 2.5 | 1.6 ± 1.4 | 1.1 ± 0.9 |
One-way anova and Tukey’s post-hoc test (*P < 0.05; **P < 0.01 compared to vehicle) indicate differences in stress responsiveness in both the 40% and 100% corticosterone animals compared to vehicle animals. Data represent the mean ± SEM of behavioural parameters scored per 10 min bins before during and after the stressor of vehicle (n = 4), 40% (n = 6) or 100% corticosterone pellets (n = 6).