Fig. (6).

Comparison of N2a cell viability (MTT assay) with the amount of ThS⁺ cells (FACS) at increasing compound concentrations. Promising compounds should show a relatively high stable cell viability value over a broad concentration range whereas the amount of ThS⁺ cells should drop already at low compound concentrations (A). By this criterion, compound ID220149 (B) is the best inhibitor in this series wereas ID220218 (C) and ID220255 (D) show less effectivity and drug-like performance. Note: The MTT value in the untreated sample (without compound) is defined as 100% cell viability. The amount of ThS⁺ cells in the Tau-induced control is normalized to 100%.