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. 2016 Aug 8;11(8):e0160659. doi: 10.1371/journal.pone.0160659

Table 1. Ingredients and chemical composition of experimental diets (total-mixed ration, dry matter basis)1.

Items Prepartum Postpartum
Ingredients, %
 Corn silage 41.94 28.19
 Alfalfa hay 0 10.25
 Wheat straw 23.48 10.25
 Corn grain 17.28 24.91
 Wheat bran 4.86 8.71
 Soybean meal 5.04 7.79
 Cottonseed meal 5.28 7.18
 Limestone 0.66 1.33
 Sodium bicarbonate 0.38 0.50
 Calcium phosphate 0.34 0.12
 Salt 0.23 0.30
 Magnesium oxide 0.17 0.15
 Vitamin and mineral premix2 0.34 0.32
Chemical composition, %
 CP 11.92 15.02
 EE 3.77 4.40
 Starch 26.48 29.13
 Ash 6.81 5.27
 NDF 45.32 36.04
 ADF 27.32 21.97
 Ca 0.51 o.78
 P 0.45 0.45
 Lys: Met3 3.13: 1 3.15: 1
 NEL, Mcal/kg dry matter3 1.51 1.69

1 CP = crude protein, EE = ether extracts, NDF = neutral detergent fiber, ADF = acid detergent fiber, Ca = calcium, P = phosphorus, Lys: Met = the ratio of lysine to methionine, and NEL = net energy for lactation.

2 Vitamin and mineral premix (per kilogram of total-mixed ration, dry matter basis): 10.5 mg Cu, 9.80 mg Zn, 12.00 mg Mn, 0.11 mg Co, 0.32 mg I, 0.15 mg Se, 2,500 IU vitamin A, 500 IU vitamin D3, and 40 IU vitamin E for pregnant cows; 6.4 mg Cu, 12.80 mg Zn, 15.20 mg Mn, 0.09 mg Co, 0.60 mg I, 0.17 mg Se, 2,500 IU vitamin A, 500 IU vitamin D3, and 70 IU vitamin E for lactating cows.

3 Lys: Met and NEL were calculated and estimated using Cornell-Penn-Miner Dairy (CPM-Dairy, version 3.0.8.1) software according to the NRC 2001 model.