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. 2016 Aug 8;11(8):e0160783. doi: 10.1371/journal.pone.0160783

Fig 3. P53 overexpression prevents TL-induced mitochondrial dysfunction in HCT116 p53-/- cells.

Fig 3

(A-B) HCT116 p53-/- cells were transiently transfected with control vector or p53 expression plasmid. After 48 hours of transfection, cells were stimulated with vehicle (DMSO) or TL (25 nM for 6 hours) before subjected for OCR analysis using Seahorse Bioscience Extra Cellular Flux analyzer. In similar experimental conditions, p53 and control vector transfected cells stimulated with DMSO or TL were also analyzed for (C) ATP level, (D) mitochondrial ROS production (MitoSOX) and (E) Mitochondrial membrane potential (ΔΨ). The data was presented as fold change over DMSO-treated vector-transfected cells. (F) P53 renders TL-mediated cell death in HCT 53-/- cells. Cell viability was assessed based on nuclear DNA content by CyQount Cell proliferation assay kit. The data was presented as %viable cells over vector-transfected DMSO-treated cells (mean±SD; *p<0.05, **p<0.001; n = 3). (G) Protein lysate from above experiment were subjected for immunoblotting for P53 to assess the P53 overexpression in HCT116 p53 -/- cells.